Question 1

The first cloned cat, called Carbon Copy, was a calico, but she looked significantly different from her female parent. Which of the following best explains the reason for the differences?

Accepted Answer

A)   The cloning was done poorly and some contaminating cat DNA became part of Carbon Copy's genome.
B)   Fur color genes in cats are determined by differential acetylation patterns that reset when cloned.
C)   Cloned animals have been found to have a higher frequency of transposon activation.
D)   X chromosome inactivation in the embryo is random and produces different patterns.E) A) and D)
F) A) and C)

Question 2

Plants are more readily manipulated by genetic engineering than are animals because ________.

Accepted Answer

A)   plant genes do not contain introns
B)   more vectors are available for transferring recombinant DNA into plant cells
C)   a somatic plant cell can often give rise to a complete plant
D)   plant cells have larger nucleiE) C) and D)
F) All of the above

Question 3

When attempting to introduce a particular fragment of DNA into the genome of an animal cell which of the following methods would be most successful?

Accepted Answer

A)   CRISPR/Cas9 recombination
B)   in vitro hybridization of the DNA with the cell's genome
C)   infecting the mouse cell with a Ti plasmid
D)   RNA-seq followed by genetic profilingE) A) and D)
F) B) and C)

Question 4

A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird) . To compare a specific region of the DNA from a sample with DNA from living birds, which of the following would be most useful for increasing the amount of dodo DNA available for testing?

Accepted Answer

A)   SNP analysis
B)   polymerase chain reaction (PCR) 
C)   electroporation
D)   gel electrophoresisE) C) and D)
F) B) and C)

Question 5

Which of the following characteristics of Taq polymerase make it useful in the PCR process?

Accepted Answer

A)   It is heat stable and can withstand the heating step of PCR.
B)   It is isolated from bacteria so there is a large supply.
C)   It binds more readily than other polymerases to the primers.
D)   It has regions that are complementary to the primers.E) A) and D)
F) A) and B)

Question 6

Which of the following statements best explain why retroviral vectors for gene therapy may increase the patient's risk of developing cancer?

Accepted Answer

A)   Retroviral vectors introduce proteins from the virus that alters control of the host cell's cell cycle.
B)   Retroviral vectors do not express the genes that were introduced into a patient's cells.
C)   Activating transcription of the retroviral vector's genes may result in spread of the virus throughout the body.
D)   Integration of the retroviral vector's DNA into the genome may misregulate the expression of genes near the integration site.E) C) and D)
F) B) and C)

Question 7

Which of the following is true of cDNA produced using human brain tissue as the starting material?

Accepted Answer

A)   The procedure to make it requires amplification by the polymerase chain reaction.
B)   It is produced from pre-mRNA using reverse transcriptase.
C)   It can be labeled and used as a probe to detect genes expressed in the brain.
D)   It includes the introns of the pre-mRNA.E) All of the above
F) A) and B)

Question 8

Being able to amplify specific DNA fragments is critical our ability to study genes because ________.

Accepted Answer

A)   before amplification, DNA fragments are likely to bind to RNA and would no longer be able to be analyzed
B)   a gene may represent only a millionth of the cell's DNA
C)   restriction enzymes (endonucleases)  cut DNA into fragments that are too small
D)   making a clone requires multiple copies of each gene per cloneE) None of the above
F) A) and D)

Question 9

Which of the following characteristics best support using yeast cells instead of bacterial cells to express mammalian proteins via cloning?

Accepted Answer

A)   They easily form colonies.
B)   They can grow on solid or liquid media.
C)   They do not have plasmids.
D)   They are eukaryotic cells.E) C) and D)
F) A) and C)

Question 10

Which of the following processes uses labeled probes to visualize the expression of genes in whole tissues and organisms?

Accepted Answer

A)   RT-PCR
B)   in situ hybridization
C)   DNA microarrays
D)   RNA interferenceE) A) and D)
F) A) and C)

Question 11

Which of the following best explains the inability of bacteria to correctly express the protein products of plasmid containing an unmodified mammalian gene?

Accepted Answer

A)   prokaryotes use a different genetic code from that of eukaryotes
B)   bacteria translate only mRNAs that have multiple messages
C)   bacteria cannot remove eukaryotic introns
D)   bacterial RNA polymerase cannot make RNA complementary to mammalian DNAE) A) and D)
F) A) and B)

Question 12

Use the figure to answer the following question.  The segment of DNA shown in the figure has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis best represents the separation and identity of these fragments?

Accepted Answer

A)  
B)  
C)  
D)  E) None of the above
F) A) and B)

Question 13

A person is heterozygous for a locus on Chromosome 1. The first copy of Chromosome 1 has the sequence 5′-AACTACGA-3′; the second copy has the sequence 5′-AACTTCGA-3′. Which of the following statements correctly describes theses sequences?

Accepted Answer

A)   They contain a SNP that may be useful for genetic mapping.
B)   They identify a location where only one copy could be cut by a restriction enzyme.
C)   They contain an STR that may be useful for forensic analysis.
D)   They may carry out RNA interference.E) A) and C)
F) A) and D)

Question 14

If a researcher is examining cells from a breast tumor biopsy which of the following microarray assay (DNA chip)  outcomes would best support a diagnosis that the tumor is cancerous?

Accepted Answer

A)   a mitochondrial encoded gene shows significantly higher expression in these cells than in liver cells
B)   the pattern of fluorescence indicates some cells are undergoing mitosis.
C)   the expression pattern in these cells differs from the pattern in non-tumor breast tissue
D)   a group of cDNAs expression matches those in colon cancer samples from the same populationE) A) and B)
F) A) and C)

Question 15

Which of the following enzymes is required to make complementary DNA (cDNA) from RNA?

Accepted Answer

A)   restriction enzymes (endonucleases) 
B)   helicase
C)   DNA ligase
D)   reverse transcriptaseE) A) and D)
F) None of the above

Question 16

The cloning of Dolly the sheep is an example of which of the following processes?

Accepted Answer

A)   replication and dedifferentiation of adult stem cells from sheep bone marrow
B)   separation of an early stage sheep blastula into separate cells, one of which was incubated in a surrogate sheep
C)   transfer of an adult cell's nucleus into an enucleated sheep egg, followed by incubation in a surrogate
D)   isolation of stem cells from a lamb embryo and production of a zygote equivalentE) A) and C)
F) B) and C)

Exam 19: Dna Technology

If a researcher is examining cells from a breast tumor biopsy which of the following microarray assay (DNA chip) outcomes would best support a diagnosis that the tumor is cancerous?

Correct Answer
verified

Which of the following processes uses labeled probes to visualize the expression of genes in whole tissues and organisms?

Correct Answer
verified

Which of the following best explains the inability of bacteria to correctly express the protein products of plasmid containing an unmodified mammalian gene?

Correct Answer
verified

A person is heterozygous for a locus on Chromosome 1. The first copy of Chromosome 1 has the sequence 5′-AACTACGA-3′; the second copy has the sequence 5′-AACTTCGA-3′. Which of the following statements correctly describes theses sequences?

Correct Answer
verified

A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird) . To compare a specific region of the DNA from a sample with DNA from living birds, which of the following would be most useful for increasing the amount of dodo DNA available for testing?

Correct Answer
verified

The cloning of Dolly the sheep is an example of which of the following processes?

Correct Answer
verified

Which of the following is true of cDNA produced using human brain tissue as the starting material?

Correct Answer
verified

The first cloned cat, called Carbon Copy, was a calico, but she looked significantly different from her female parent. Which of the following best explains the reason for the differences?

Correct Answer
verified

Which of the following characteristics best support using yeast cells instead of bacterial cells to express mammalian proteins via cloning?

Correct Answer
verified

Plants are more readily manipulated by genetic engineering than are animals because ________.

Correct Answer
verified

Which of the following enzymes is required to make complementary DNA (cDNA) from RNA?

Correct Answer
verified

Being able to amplify specific DNA fragments is critical our ability to study genes because ________.

Correct Answer
verified

Which of the following statements best explain why retroviral vectors for gene therapy may increase the patient's risk of developing cancer?

Correct Answer
verified

Which of the following characteristics of Taq polymerase make it useful in the PCR process?

Correct Answer
verified

When attempting to introduce a particular fragment of DNA into the genome of an animal cell which of the following methods would be most successful?

Correct Answer
verified

Use the figure to answer the following question. The segment of DNA shown in the figure has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis best represents the separation and identity of these fragments?

Correct Answer
verified

Exam 19: Dna Technology

If a researcher is examining cells from a breast tumor biopsy which of the following microarray assay (DNA chip) outcomes would best support a diagnosis that the tumor is cancerous?

Correct AnswerverifiedShow Answer

Which of the following processes uses labeled probes to visualize the expression of genes in whole tissues and organisms?

Correct AnswerverifiedShow Answer

Which of the following best explains the inability of bacteria to correctly express the protein products of plasmid containing an unmodified mammalian gene?

Correct AnswerverifiedShow Answer

A person is heterozygous for a locus on Chromosome 1. The first copy of Chromosome 1 has the sequence 5′-AACTACGA-3′; the second copy has the sequence 5′-AACTTCGA-3′. Which of the following statements correctly describes theses sequences?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

The cloning of Dolly the sheep is an example of which of the following processes?

Correct AnswerverifiedShow Answer

Which of the following is true of cDNA produced using human brain tissue as the starting material?

Correct AnswerverifiedShow Answer

The first cloned cat, called Carbon Copy, was a calico, but she looked significantly different from her female parent. Which of the following best explains the reason for the differences?

Correct AnswerverifiedShow Answer

Which of the following characteristics best support using yeast cells instead of bacterial cells to express mammalian proteins via cloning?

Correct AnswerverifiedShow Answer

Plants are more readily manipulated by genetic engineering than are animals because ________.

Correct AnswerverifiedShow Answer

Which of the following enzymes is required to make complementary DNA (cDNA) from RNA?

Correct AnswerverifiedShow Answer

Being able to amplify specific DNA fragments is critical our ability to study genes because ________.

Correct AnswerverifiedShow Answer

Which of the following statements best explain why retroviral vectors for gene therapy may increase the patient's risk of developing cancer?

Correct AnswerverifiedShow Answer

Which of the following characteristics of Taq polymerase make it useful in the PCR process?

Correct AnswerverifiedShow Answer

When attempting to introduce a particular fragment of DNA into the genome of an animal cell which of the following methods would be most successful?

Correct AnswerverifiedShow Answer

Use the figure to answer the following question. The segment of DNA shown in the figure has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis best represents the separation and identity of these fragments?

Correct AnswerverifiedShow Answer

Correct Answer
verified

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