Question 1

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

A)  stage
B)  condenser
C)  objective
D)  ocular
E)  nosepieceF) B) and E)
G) B) and C)

Question 2

A pure culture contains

Accepted Answer

A)  only one identified species of microorganism.
B)  only bacteria.
C)  a variety of microbes from one source.
D)  a variety of species from the same genus.E) A) and B)
F) B) and D)

Question 3

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Accepted Answer

A)  ocular;objective
B)  scanning;objective
C)  objective;ocular
D)  ocular;oil immersionE) None of the above
F) B) and C)

Question 4

A medium that is gel-like has less agar in it compared to a solid medium.

Accepted Answer

A) True 
 B)False

Question 5

One colony typically develops from the growth of several parent bacterial cells.

Accepted Answer

A) True 
 B)False

Question 6

A confocal scanning optical microscope

Accepted Answer

A)  uses ultraviolet light to form a specimen image.
B)  shows three-dimensional cell images from the cell surface to the middle of the cell.
C)  produces specimen images on electron micrographs.
D)  uses dyes that emit visible light when bombarded by ultraviolet rays.
E)  requires specimens to be stained.F) A) and B)
G) B) and E)

Question 7

Differential media result in which of the following growth characteristics?

Accepted Answer

A)  different color colonies
B)  different media color post incubation
C)  precipitates
D)  gas bubbles
E)  All of the choices are correct.F) A) and B)
G) A) and C)

Question 8

Eosin-methylene blue agar,or EMB,is a commonly used bacteriological medium in a lab,used to grow gram-negative bacteria.There are two dyes in it,eosin and methylene blue,as well as the sugar lactose.Eosin is a pH indicator that changes color when the medium is acidic,while methylene blue reduces the growth of most gram-positive bacteria.If an organism uses lactose,acid will be produced changing the pH of the medium and subsequently causing the bacterial colonies to change color.
Based upon this description,this medium is:

Accepted Answer

A)  reducing
B)  enrichment
C)  selective
D)  differential
E)  differential and selectiveF) A) and C)
G) A) and D)

Question 9

Let us say that the 100X magnification lens of your microscope has a resolving power of 0.15 microns (micrometers) .The specimen that you will be looking at has bacterial cocci the size of 0.1 microns in diameter.Which statement is correct?

Accepted Answer

A)  The cocci will be easily seen and very clear.
B)  The cocci will be fuzzy-looking and blend together.
C)  The cocci will appear much smaller than they really are because of the small resolving power.
D)  The cocci will be totally invisible.E) B) and C)
F) C) and D)

Question 10

Which type of microscope cannot image live specimens?

Accepted Answer

A)  bright-field
B)  phase-contrast
C)  dark-field
D)  electron
E)  differential interferenceF) B) and E)
G) A) and B)

Question 11

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A)  differential
B)  selective
C)  enumeration
D)  enriched
E)  reducingF) A) and C)
G) A) and E)

Question 12

The purpose of staining cells on a microscope slide is to

Accepted Answer

A)  kill them.
B)  secure them to the slide.
C)  enlarge the cells.
D)  add contrast in order to see them better.
E)  see motility.F) All of the above
G) None of the above

Question 13

What is the term for a culture made from one isolated colony?

Accepted Answer

A)  axenic
B)  mixed
C)  pure
D)  Both axenic and mixed are correct.
E)  Both axenic and pure are correct.F) C) and E)
G) None of the above

Question 14

Bacterial cultures are easily identified from their microscopic appearance.

Accepted Answer

A) True 
 B)False

Question 15

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible,is termed

Accepted Answer

A)  complex.
B)  reducing.
C)  enriched.
D)  synthetic.E) A) and B)
F) B) and C)

Question 16

The Five I's of studying microorganisms include all of the following except

Accepted Answer

A)  inoculation.
B)  incubation.
C)  infection.
D)  isolation.
E)  identification.F) A) and D)
G) D) and E)

Question 17

Some microbes are not capable of growing on artificial media.

Accepted Answer

A) True 
 B)False

Question 18

A reducing medium is used to culture

Accepted Answer

A)  fastidious organisms.
B)  aerobic organisms.
C)  anaerobic organisms.
D)  any pathogenic organisms.E) None of the above
F) All of the above

Question 19

Which of the following controls the amount of light entering the condenser?

Accepted Answer

A)  objective lens
B)  ocular lens
C)  condenser
D)  body
E)  iris diaphragmF) C) and D)
G) All of the above

Question 20

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Accepted Answer

A)  NaCl
B)  sheep red blood cells
C)  bile salts
D)  thioglycollic acid
E)  peptoneF) None of the above
G) C) and E)

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

The Five I's of studying microorganisms include all of the following except

Correct Answer
verified

Some microbes are not capable of growing on artificial media.

Correct Answer
verified

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Correct Answer
verified

Which type of microscope cannot image live specimens?

Correct Answer
verified

Which of the following controls the amount of light entering the condenser?

Correct Answer
verified

The _____ of the microscope holds and allows selection of the objective lenses.

Correct Answer
verified

A confocal scanning optical microscope

Correct Answer
verified

Bacterial cultures are easily identified from their microscopic appearance.

Correct Answer
verified

A pure culture contains

Correct Answer
verified

The purpose of staining cells on a microscope slide is to

Correct Answer
verified

What is the term for a culture made from one isolated colony?

Correct Answer
verified

A reducing medium is used to culture

Correct Answer
verified

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct Answer
verified

One colony typically develops from the growth of several parent bacterial cells.

Correct Answer
verified

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible,is termed

Correct Answer
verified

Differential media result in which of the following growth characteristics?

Correct Answer
verified

Let us say that the 100X magnification lens of your microscope has a resolving power of 0.15 microns (micrometers) .The specimen that you will be looking at has bacterial cocci the size of 0.1 microns in diameter.Which statement is correct?

Correct Answer
verified

A medium that is gel-like has less agar in it compared to a solid medium.

Correct Answer
verified

Correct Answer
verified

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Correct Answer
verified

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

The Five I's of studying microorganisms include all of the following except

Correct AnswerverifiedShow Answer

Some microbes are not capable of growing on artificial media.

Correct AnswerverifiedShow Answer

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Correct AnswerverifiedShow Answer

Which type of microscope cannot image live specimens?

Correct AnswerverifiedShow Answer

Which of the following controls the amount of light entering the condenser?

Correct AnswerverifiedShow Answer

The _____ of the microscope holds and allows selection of the objective lenses.

Correct AnswerverifiedShow Answer

A confocal scanning optical microscope

Correct AnswerverifiedShow Answer

Bacterial cultures are easily identified from their microscopic appearance.

Correct AnswerverifiedShow Answer

A pure culture contains

Correct AnswerverifiedShow Answer

The purpose of staining cells on a microscope slide is to

Correct AnswerverifiedShow Answer

What is the term for a culture made from one isolated colony?

Correct AnswerverifiedShow Answer

A reducing medium is used to culture

Correct AnswerverifiedShow Answer

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct AnswerverifiedShow Answer

One colony typically develops from the growth of several parent bacterial cells.

Correct AnswerverifiedShow Answer

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible,is termed

Correct AnswerverifiedShow Answer

Differential media result in which of the following growth characteristics?

Correct AnswerverifiedShow Answer

Let us say that the 100X magnification lens of your microscope has a resolving power of 0.15 microns (micrometers) .The specimen that you will be looking at has bacterial cocci the size of 0.1 microns in diameter.Which statement is correct?

Correct AnswerverifiedShow Answer

A medium that is gel-like has less agar in it compared to a solid medium.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Correct AnswerverifiedShow Answer

Correct Answer
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Correct Answer
verified

Correct Answer
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verified

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct Answer
verified

Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
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